Sequencing
DNA sequencing services are currently provided using the Applied Biosystems 3730xl DNA Analyzer. The ABI machines utilize a one-capillary cycle sequencing process with refined, four-dye fluorescent labeling methods and a real-time scanning detector. The biochemistry of one-lane sequencing is similar to Sanger-based methods.
The JHU Finch server automatically processes data after electrophoresis is complete and provides quality assessment for each sample. Data is available for download, viewing, and editing.
The service provides standard sequencing primers for several vectors. Custom primers are provided by the user or can be synthesized in our facility.