Tetraethylthiuram disulfide (TETD) in acetronitrile is available for synthesizing internucleotide analog, phosphorothioate oligonucleotides. Even after long exposure to the TETD reagent, analysis of oligonucleotides by enzymatic digestion shows no detectable base modifications.
TETD converts internucleotide cyanoethyl phosphite to the phosphorothioate triester.
