Aminolink 2
Description
Aminolink 2 affords a convenient method for the introduction of an aliphatic primary amine at the 5' end of oligonucleotides. This amino can react to form oligonucleotide conjugates with a variety of substrates such as biotin, fluorescent dyes, EDTA, or alkaline phosphatase. Applications include:
- Non radio-labeled hybridization probes
- Sequence specific cleavage of single stranded DNA
- Automated DNA sequencing
- Affinity chromatography
Aminolink 2 offers many advantages when used in applications like those above. Because it is a phosphoramidite, it reacts like conventional nucleoside phosphoramidites, i.e., the compound is dissolved in anhydrous acetronitrile and placed on any of the extra phosphoramidite ports. It is activated with tetrazole and couples with the same high efficiency found in standard oligonucleotide synthesis.
- Solution stability is comparable to nucleoside phosphoramidites (at least 2 weeks)
- No special cycles or protocols required regardless of instrument model
- Labeling at 5'-terminal amine does not interfere with hybridization
- Aminolinked oligonucleotides can be easily labeled with a variety of commercially available reagents such as fluorescein isothiocyanate and biotin NHS ester
Chemical Structure
Aminolink is a registered trademark of Applied Biosystems.