DNA Analysis Facility

FAQ

  1. How should I provide my template and primer?

    Please provide your pre-mixed sample containing 6 µl of template and 6 µl of primer, at the following concentrations:

    Primer: 1µM.
    PCR products: 3.0 ng/µl/100 bp.
    Plasmid DNA: 0.2µg/µl.
    BAC/PAC DNA: please contact the lab.

  2. How do I place a sequencing order?

    The JHU Finch Server manages all orders and data. To place an order and view your data, you must have a username and password. If you need a JHU Finch server login, please call us at (410) 614-6548 and we will be happy to assist you. Please check our protocols section for detailed help with placing a sequencing request.

    Please remember, in order to utilize all of the features of the server, including order notifications and data tracking, each individual in your lab should have their own login.

  3. When will my data be ready?

    Results for sequencing samples dropped off by 2:00 PM will generally be available by 10:30 AM the following day (contingent on run space). You will receive an email when your data is completed.

  4. Where do I drop off my samples?

    Blalock 1005: between 8 AM- 4 PM. There is also a drop-box available for after-hours.

    CRB II Room LB06: Samples dropped off here by 1:30 PM will be picked up and processed the next work day as capacity permits.

    Bayview Campus: In room 3A2 of the Asthma and Allergy building you will find a drop-box in which to drop off your samples. Samples will be picked up from here daily.

  5. How should I submit my samples?

    Tubes: due to storage issues, we will accept only 1.5 ml eppendorf tubes. Plates: 24 samples or more may be submitted on plates with the requirement that all consecutive wells are filled in a top to bottom, left to right format (i.e. A1-H1; A2-H2, etc.).

    Labeling: Your tubes need to be labeled with your sample request ID and matching Finch server sample ID.

    Note: Any sample that is improperly labeled, unreadable, or any sample sets organized in a way that we feel may increase the risk of error during handling in the DNA Analysis Facility may be rejected. Improperly labeled samples will be returned.

  6. How much does sequencing cost?

    Single template/primer: $10 (pre-mixed). An additional $5 will be charged for samples that are not pre-mixed. Standard primers are available for you at Blalock 1005.

    80-96 samples in a 96-well plate: $768